碘化丙啶
| 中文名稱 | 碘化丙啶 |
|---|---|
| 中文同義詞 | 碘化丙啶;(碘化丙碇)PI 10MG;碘丙啶;碘化丙啶(>95% (HPLC),BC);L-Α-磷脂酰肌醇 銨鹽;1,2-二?;?SN-甘油-3-磷酸基-(1-D-MYO-肌醇) 鉀銨鹽;L-Α-磷脂酰肌醇 銨鹽 來源于牛肝臟;(碘化丙碇)PI |
| 英文名稱 | Propidium iodide |
| 英文同義詞 | 3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE;3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod;3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi;PHENANTHRIDINIUM, 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYL-, DIIODIDE |
| CAS號(hào) | 25535-16-4 |
| 分子式 | C27H34IN4+ |
| 分子量 | 541.5 |
| EINECS號(hào) | 247-081-0 |
| 相關(guān)類別 | 細(xì)胞生物學(xué);熒光染料;小分子抑制劑;生物活性小分子;細(xì)胞信號(hào)和神經(jīng)生物學(xué);小分子抑制劑,天然產(chǎn)物;色素類;細(xì)胞生物學(xué)試劑;化學(xué)試劑;Aromatics Compounds;Aromatics;Intermediates & Fine Chemicals;Pharmaceuticals;marker;Inhibitors;Phenanthridine;生化試劑;生物化工;有機(jī)化學(xué);合成材料中間體;合成中間體;通用生化試劑-生物染色劑與化學(xué)指示劑;試劑盒-Elisa試劑盒;對(duì)照品 |
| Mol文件 | 25535-16-4.mol |
| 結(jié)構(gòu)式 | ![]() |
碘化丙啶 性質(zhì)
| 熔點(diǎn) | 220-225 °C (dec.) (lit.) |
|---|---|
| 密度 | 1.5072 (estimate) |
| 儲(chǔ)存條件 | 2-8°C |
| 溶解度 | 難溶于水 |
| 形態(tài) | 片狀粉末 |
| 顏色 | 深紅 |
| 外觀 | Red solid or powder |
| 水溶解性 | Soluble in water at 1mg/ml |
| 敏感性 | Light Sensitive & Hygroscopic |
| 最大波長(zhǎng)(λmax) | 493 nm, 535 nm |
| 激發(fā)峰(Ex)/發(fā)射峰(Em) | 535/617 nm (and DNA) |
| ε(消光系數(shù)) | 5400 cm?1M?1 (water/DNA) |
| BRN | 3843838 |
| 生物領(lǐng)域應(yīng)用 | Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer |
| 主要應(yīng)用 | 細(xì)胞凋亡檢測(cè);細(xì)胞周期分析 |
| InChIKey | ISKCJFFZOPXOFC-UHFFFAOYSA-N |
| SMILES | C([N+]1=C2C=C(N)C=CC2=C2C=CC(N)=CC2=C1C1C=CC=CC=1)CC[N+](C)(CC)CC.[I-] |
| CAS 數(shù)據(jù)庫 | 25535-16-4(CAS DataBase Reference) |
碘化丙啶是一種可對(duì)DNA染色的細(xì)胞核染色試劑,常用于細(xì)胞凋亡檢測(cè),英文全稱是Propidium Iodide。它是一種溴化乙啶的類似物,在嵌入雙鏈DNA后釋放紅色熒光。
碘化丙啶(Propidium iodide)是一種實(shí)驗(yàn)室常用的紅色熒光染料,常用于對(duì)染(counterstain)細(xì)胞核和染色體。由于它不能透過活細(xì)胞細(xì)胞膜,碘化丙啶也常用于染色鑒別細(xì)胞群中的死細(xì)胞。碘化丙啶本身熒光很弱,在沒有DNA存在的水溶液中,碘化丙啶只有很弱的493nm的激發(fā)峰和636nm的發(fā)射峰,但當(dāng)加入DNA后,碘化丙啶嵌入DNA雙鏈,并熒光強(qiáng)度增加20-30倍,同時(shí)激發(fā)峰紅移到535nm,發(fā)射峰藍(lán)移到617nm。
盡管碘化丙啶的消光系數(shù)較低,但是它的斯托克斯位移很大,可讓同時(shí)檢測(cè)碘化丙啶熒光和熒光素類紅色熒光,常常用于流式細(xì)胞實(shí)驗(yàn)中檢測(cè)細(xì)胞凋亡,細(xì)胞周期等。碘化丙啶也可以用于熒光顯微鏡,共聚焦顯微鏡等中的細(xì)胞核染色實(shí)驗(yàn)。Propidium Iodide 是可用于細(xì)胞染色的紅色熒光染料。
Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI + -pycnotic nuclei of degenerating neurons . Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes.
ex/em : 535/617 nm (和DNA)
消光系數(shù)(ε) : 5400 cm?1M?1 (水/DNA)
安全信息
| 危險(xiǎn)品標(biāo)志 | Xi,Xn |
|---|---|
| 危險(xiǎn)類別碼 | 36/37/38-68-67-48/20/22-40-36/38-22-20-63 |
| 安全說明 | 26-36-22-36/37 |
| WGK Germany | 3 |
| RTECS號(hào) | SF7949600 |
| F | 3-8-10-23 |
| 海關(guān)編碼 | 29339900 |
| 存儲(chǔ)類別 | 12 - 不可燃性液體 |
| 提供商 | 語言 |
|---|---|
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英文
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| 更新日期 | 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | CAS號(hào) | 包裝 | 價(jià)格 |
|---|---|---|---|---|---|
| 2026/07/06 | J66764 | 碘化丙啶 Propidium iodide | 25535-16-4 | 100mg | 999999元 |
| 2026/07/06 | 44030 | 碘化丙啶 Propidium iodide, 95% | 25535-16-4 | 100mg | 2046元 |
