Sequencing Grade Modified Trypsin
Catalog Number:SRT0201
CAS:9002-07-7
EC:3.4.21.4
Storage temperature: 2-8℃
Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin
1.DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
2.MAIN FEATURES
3.RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.
It is no activity loss after 5 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
- Sequence grade recombinant protein: high specificity,good stability.
- Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
- Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
- Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.
7.RELATED PRODUCTS
Recombinant enterokinase
Recombinant V8 protease
Sequencing grade chymotrypsin
Sequencing grade carboxypeptidase B
關(guān)鍵字： Trypsin； Recombinant Trypsin；Sequencing Grade Modified trypsin
上海雅心生物技術(shù)有限公司主要從事基因工程蛋白酶的研發(fā)和生產(chǎn)，目前的主要品種為基因工程羧肽酶B、基因工程胰蛋白酶和基因工程脂肪酶A；為本公司自主研發(fā)，擁有自主知識(shí)產(chǎn)權(quán)。公司擁有自組研發(fā)團(tuán)隊(duì)，擁有世界先進(jìn)的重組生物技術(shù)；自主研發(fā)的重組生物用酶為世界上生物用酶的首創(chuàng)！
基因工程羧肽酶B，基因工程胰蛋白酶和基因工程脂肪酶A都是由基因工程方法生產(chǎn)，純度高，不含雜酶，無羧肽酶A、糜蛋白酶等其他蛋白酶活性；無動(dòng)物源性，出口產(chǎn)品無需進(jìn)行終產(chǎn)品的動(dòng)物源性檢驗(yàn)。制品中不含蛋白酶抑制劑，產(chǎn)品安全。
公司秉承“科技致勝”的理念，堅(jiān)持“提供高純度重組蛋白酶”，并愿與廣大同行交流，有需求的客戶提供優(yōu)質(zhì)的服務(wù)！
產(chǎn)品經(jīng)多