一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> International Journal of Molecular Sciences >>article
International Journal of Molecular Sciences

International Journal of Molecular Sciences

IF: 5.6
Download PDF

CDC20 Holds Novel Regulation Mechanism in RPA1 during Different Stages of DNA Damage to Induce Radio-Chemoresistance.

Published:1 August 2024 DOI:
Yang Gao, Pengbo Wen, Chenran Shao, Cheng Ye, Yuji Chen, Junyu You, Zhongjing Su

Abstract

Targeting CDC20 can enhance the radiosensitivity of tumor cells, but the function and mechanism of CDC20 on DNA damage repair response remains vague. To examine that issue, tumor cell lines, including KYSE200, KYSE450, and HCT116, were utilized to detect the expression, function, and underlying mechanism of CDC20 in radio-chemoresistance. Western blot and immunofluorescence staining were employed to confirm CDC20 expression and location, and radiation could upregulate the expression of CDC20 in the cell nucleus. The homologous recombination (HR) and non-homologous end joining (NHEJ) reporter gene systems were utilized to explore the impact of CDC20 on DNA damage repair, indicating that CDC20 could promote HR repair and radio/chemo-resistance. In the early stages of DNA damage, CDC20 stabilizes the RPA1 protein through protein-protein interactions, activating the ATR-mediated signaling cascade, thereby aiding in genomic repair. In the later stages, CDC20 assists in the subsequent steps of damage repair by the ubiquitin-mediated degradation of RPA1. CCK-8 and colony formation assay were used to detect the function of CDC20 in cell vitality and proliferation, and targeting CDC20 can exacerbate the increase in DNA damage levels caused by cisplatin or etoposide. A tumor xenograft model was conducted in BALB/c-nu/nu mice to confirm the function of CDC20 in vivo, confirming the in vitro results. In conclusion, this study provides further validation of the potential clinical significance of CDC20 as a strategy to overcome radio-chemoresistance via uncovering a novel role of CDC20 in regulating RPA1 during DNA damage repair.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
MG-132 133407-82-6 C26H41N3O5 426 suppliers $11.00-$9807.90
MG-132 133407-82-6 C26H41N3O5 426 suppliers $11.00-$9807.90
MG-132 133407-82-6 C26H41N3O5 426 suppliers $11.00-$9807.90
MG-132 133407-82-6 C26H41N3O5 426 suppliers $11.00-$9807.90

Similar articles

IF:1.6

Maslinic Acid Induces DNA Damage and Impairs DNA Repair in Human Cervical Cancer HeLa Cells.

Anticancer research Kung-Wen Lu, Mei-Due Yang,etc Published: 1 December 2020
IF:2.9

Theoretical study on catalyzed selective photoreduction mechanism for 4-bromobenzaldehyde in two different solvents

Physical Chemistry Chemical Physics Xiao Huang, Liang Peng,etc Published: 1 July 2015
楚雄市| 苏尼特左旗| 沂南县| 都安| 嘉兴市| 河东区| 淮阳县| 定襄县| 稻城县| 景东| 西畴县| 高清| 社会| 大连市| 巢湖市| 大埔县| 英超| 肃北| 哈密市| 葫芦岛市| 运城市| 锡林浩特市| 开封县| 天峨县| 家居| 岚皋县| 军事| 思南县| 松桃| 城步| 化隆| 玉林市| 泌阳县| 拜泉县| 浮山县| 富平县| 沾化县| 扶沟县| 嘉峪关市| 拜泉县| 长宁县|