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ACS Sensors

ACS Sensors

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Ultrasensitive Electrochemical Vertical Flow Immunoassay for Rapid and Simultaneous Detection of Interleukin-6 and Procalcitonin

Published:5 December 2024 DOI: 10.1021/acssensors.4c02257
Xudong Ying, Wenxuan Fu, Min Qi, Lin Zhou, Wei Li, Bin Su

Abstract

Rapid and multiplexed detection of biomarkers plays an indispensable role in disease diagnosis. Although paper-based lateral flow immunoassays have been widely used in this field, the speediness and throughput are still challenging issues. Herein, an electrochemical vertical flow immunoassay device (eVFID) is fabricated for rapid, ultrasensitive, and multiplexed detection of inflammatory biomarkers. Working electrodes with excellent electrochemical performance and permeability properties were directly fabricated on the nitrocellulose membrane to enable both the vertical flow of the sample solution and electrochemical detection. This vertical configuration can remarkably improve the speediness of the immunoassay and effectively inhibit the cross-talk reactions among immunomolecules, thus allowing rapid and simultaneous detection of multiplexed biomarkers. Furthermore, a signal amplification strategy based on horseradish peroxidase and tetramethylbenzidine was integrated into the eVFID to substantially increase the sensitivity of the electrochemical detection. A low limit of detection of 0.1 and 0.22 pg mL–1 was obtained for two low-abundance inflammatory biomarkers, interleukin-6 (IL-6) and procalcitonin (PCT), respectively. Finally, using a two-channel eVFID, simultaneous detection of IL-6 and PCT in human plasma samples was successfully realized within 5 min. We believe that the eVFID holds great promise for speedy and high-throughput biomarker detection at the point of care.

Substances (5)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Polysorbate 20 9005-64-5 C26H50O10 1056 suppliers $9.00-$6080.49
Bovine albumin 9048-46-8 N/A 915 suppliers $15.00-$18680.00
Potassium hydroxide 1310-58-3 KOH 841 suppliers $10.00-$8240.00
Trehalose 99-20-7 C12H22O11 654 suppliers $6.00-$3217.55
potassium ferricyanide 13746-66-2 C6FeN6.3K 525 suppliers $7.62-$15160.00
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