一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Phytomedicine >>article
Phytomedicine

Phytomedicine

IF: 6.7
Download PDF

Stigmasterol from Prunella vulgaris L. Alleviates LPS-induced mammary gland injury by inhibiting inflammation and ferroptosis

Published:1 February 2025 DOI: 10.1016/j.phymed.2025.156362 PMID: 39809030
Mingyang Sun , Dianwen Xu , Dianfeng Liu , Xin Ran , Feng Li , Jiaxin Wang , Yusong Ge , Yuhao Liu , Wenjin Guo , Juxiong Liu , Yu Cao , Shoupeng Fu

Abstract

Background

Dairy mastitis, a prevalent condition affecting dairy cattle, represents a significant challenge to both animal welfare and the quality of dairy products. However, current treatment options remain limited. Stigmasterol (ST) is a bioactive component of Prunella vulgaris L. (PV) with various pharmacological functions such as anti-inflammatory and anti-oxidation. At present, the specific effects and underlying mechanisms of PV and ST on dairy mastitis are still not fully understood.

Purpose

The aim of this research was to evaluate the pharmacological effects of PV and its active component ST on lipopolysaccharide (LPS) -stimulated bovine mammary epithelial cells (BMECs) and a mouse mastitis model, and to elucidate the possible mechanisms of action.

Methods

UPLC-Q-TOF-MS/MS was employed to identify the constituents of PV. BMECs and mice were used to establish in vitro and in vivo models of mastitis. Western Blotting, RT-qPCR, immunofluorescence and other techniques were used to explore the effects of PV and ST on inflammatory factors, blood-milk barrier integrity, ferroptosis related indicators and their potential molecular mechanisms.

Results

PV significantly attenuated the production of inflammatory mediators by LPS-stimulated BMECs. Subsequently, ST was found to be a potent anti-inflammatory agent in PV by inhibiting TLR4/NF-κB signaling pathway. This inhibition inhibits the myosin light chain (MLC)/MLC kinase signaling cascade and alleviates blood-milk barrier (BMB) disruption in BMECs. In addition, ferroptosis occurred in BMECs after LPS stimulation, and ST inhibited ferroptosis by stimulating Nrf2/GPX4 signaling pathway. Treatment of BMECs with the Nrf2 inhibitor ML385 significantly attenuated the therapeutic effect of ST. In vivo experiments further confirmed that both PV and ST attenuated LPS-induced breast tissue damage while reducing ferroptosis levels and restoring BMB.

Conclusion

ST from PV exhibits substantial anti-inflammatory properties and is a promising candidate for the treatment of dairy mastitis.

Substances (16)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Rutin 153-18-4 C27H30O16 900 suppliers $6.00-$1900.00
Rutin 153-18-4 C27H30O16 900 suppliers $6.00-$1900.00
Rutin 153-18-4 C27H30O16 900 suppliers $6.00-$1900.00
Rutin 153-18-4 C27H30O16 900 suppliers $6.00-$1900.00
LINOELAIDIC ACID 506-21-8 C18H32O2 89 suppliers $20.00-$155030.00
LINOELAIDIC ACID 506-21-8 C18H32O2 89 suppliers $20.00-$155030.00
LINOELAIDIC ACID 506-21-8 C18H32O2 89 suppliers $20.00-$155030.00
LINOELAIDIC ACID 506-21-8 C18H32O2 89 suppliers $20.00-$155030.00
Rutin 153-18-4 - Inquiry
Rutin 153-18-4 - Inquiry
临沭县| 兰考县| 滦南县| 红原县| 萨嘎县| 渭源县| 兴国县| 乌拉特前旗| 衡南县| 通海县| 额敏县| 西充县| 右玉县| 南乐县| 平度市| 威宁| 桃江县| 手游| 宝应县| 波密县| 柳河县| 凤台县| 渝中区| 专栏| 福建省| 乐山市| 宁陵县| 隆昌县| 余江县| 石首市| 饶平县| 合山市| 贵阳市| 万宁市| 兴隆县| 本溪市| 永宁县| 英山县| 黑河市| 满城县| 丁青县|