一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Human & Experimental Toxicology >>article
Human & Experimental Toxicology

Human & Experimental Toxicology

IF: 2.7
Download PDF

Glyoxal toxicity in isolated rat liver mitochondria.

Published:1 May 2018 DOI: 10.1177/0960327117715900 PMID: 28639457
M Goudarzi, H Kalantari, M Rezaei

Abstract

Glyoxal is a physiological metabolite formed by lipid peroxidation, ascorbate autoxidation, oxidative degradation of glucose, and degradation of glycated proteins. Glyoxal has been linked to oxidative stress and can cause a number of cellular damages, including covalent modification of amino and thiol groups of proteins to form advanced glycation end products. However, the mechanism of glyoxal toxicity has not been fully understood. In this study, we have focused on glyoxal toxicity in isolated rat liver mitochondria. Isolated mitochondria (0.5 mg protein per milliliter) were prepared from the Wistar rat liver using differential centrifugation and incubated with various concentrations of glyoxal (1, 2.5, 5, 7.5, and 10 mM) for 30 min. The activity of mitochondrial complex II was determined by measurement of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) conversion. The mitochondrial membrane potential (MMP), lipid peroxidation (MDA), reactive oxygen species (ROS) formation, glutathione (GSH) content, and protein carbonylation were also assessed. After an incubation of isolated liver mitochondria with glyoxal, disrupted electron transport chain, increased mitochondrial ROS formation, lipid peroxidation, mitochondrial membrane damage, GSH oxidation, and protein carbonylation ensued as compared to the control group ( p < 0.05). Glyoxal toxicity in isolated rat liver mitochondria was dose-dependent. In conclusion, glyoxal impaired the electron transport chain, which is the cause of increased ROS and MDA production, depletion of GSH, and disruption of MMP. Mitotoxicity of glyoxal might be related to the pathomechanisms involved in diabetes and its complications.

Substances (5)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Glutathione 70-18-8 C10H17N3O6S 1413 suppliers $6.00-$3580.00
Glyoxal 107-22-2 C2H2O2 669 suppliers $7.62-$1720.00
Thiazolyl Blue 298-93-1 C18H16N5S.Br 607 suppliers $7.00-$1850.00
4,4'-Methylenedianiline 101-77-9 C13H14N2 413 suppliers $15.00-$1559.25
ROS 37 suppliers Inquiry

Similar articles

IF:6.7

Phytochemicals and mitochondria: Therapeutic allies against gastric cancer

Phytomedicine Maoyuan Zhao , Yi Yang ,etc Published: 1 February 2023
IF:3.5

Quinoline Endoperoxides for Mitochondria Targeted Singlet Oxygen Delivery

Chemistry - An Asian Journal Yu Si, Lei Wang,etc Published: 29 November 2024
永泰县| 都安| 迭部县| 绥阳县| 萨嘎县| 郧西县| 郑州市| 邹城市| 长治市| 安溪县| 玛多县| 农安县| 凭祥市| 深水埗区| 喜德县| 榆树市| 嘉祥县| 邵东县| 天镇县| 多伦县| 宿松县| 吉林市| 辽源市| 二手房| 方正县| 扎鲁特旗| 南平市| 铅山县| 九江市| 清水河县| 安平县| 大理市| 泌阳县| 桓台县| 清苑县| 澄江县| 镇江市| 永寿县| 大洼县| 汉川市| 沙田区|