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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Argatroban Impurity 26 Argatroban Impurity 26 189264-02-6 C23H36N6O5S
ROC-325 ROC-325 1859141-26-6 C28H27ClN4OS
Pitavastatin Impurity 2 Pitavastatin Impurity 2 1187966-93-3 C25H22FNO4
LPC(15:0) LPC(15:0) 1357147-16-0 C23H48NO7P
LCZ 696  Impurity B LCZ 696 Impurity B 766480-48-2 C24H29NO5
LCZ 696 Impurity C LCZ 696 Impurity C 149709-63-7 C24H29NO5
Canagliflozin α Isomer Canagliflozin α Isomer 1589590-87-3 C24H25FO5S
5,5'-diMethoxycarbonyl-2,2'-bipyridine 5,5'-diMethoxycarbonyl-2,2'-bipyridine 1762-45-4 C14H12N2O4
Vildagliptin IMpurity 2 (Mixture of DiastereoMers) Vildagliptin IMpurity 2 (Mixture of DiastereoMers) 1036959-23-5 C24H33N5O3
ahu377  isomer 2 ahu377 isomer 2 761373-05-1 C24H29NO5
Apixaban Dehydro Impurity Apixaban Dehydro Impurity 1074549-89-5 C25H23N5O4
9-CIS-RETINOIC ACID 9-CIS-RETINOIC ACID 5300-03-8 C20H28O2
Ozagrel Impurity 7 Ozagrel Impurity 7 883903-91-1 C23H21N2NaO4
N-Des acetyl impurity N-Des acetyl impurity
ALPHA-(-)-THUJONE ALPHA-(-)-THUJONE 546-80-5 C10H16O
Peramivir Impurity 6 Peramivir Impurity 6 2124296-37-1 C15H28N4O4
BRD0705 BRD0705 2056261-41-5 C20H23N3O
Rasagiline Impurity 2 Rasagiline Impurity 2 200628-33-7 C16H18ClN
Bortezomib Impurity WQ Bortezomib Impurity WQ
6-alfa-Hydroxy Mometasone Furoate 6-alfa-Hydroxy Mometasone Furoate 134429-33-7 C27H30Cl2O7
Cefdinir Impurity Cefdinir Impurity
Plerixafor Impurity 7 Plerixafor Impurity 7 117152-72-4 C24H36N4O4S2
Clindamycin Phosphate EP Impurity I Clindamycin Phosphate EP Impurity I
Cilastatin EP Impurity C Cilastatin EP Impurity C
Atracurium EP Impurity G Atracurium EP Impurity G
(R)-N-((S)-1-amino-1-oxobutan-2-yl)-3-(bromomethyl)hexanamide (R)-N-((S)-1-amino-1-oxobutan-2-yl)-3-(bromomethyl)hexanamide 2242647-55-6 C11H21BrN2O2
(2S)-2[[(1S)-1-(ETHOXYCARBONYL)-3-PHENYLPROPYL]AMINO]PROPANOIC ACID ETHYL ESTER HYDROCHLORIDE (2S)-2[[(1S)-1-(ETHOXYCARBONYL)-3-PHENYLPROPYL]AMINO]PROPANOIC ACID ETHYL ESTER HYDROCHLORIDE 2378310-70-2 C17H26ClNO4
Acetamide, N-(2-benzoyl-4-nitrophenyl)-2,2-dichloro- Acetamide, N-(2-benzoyl-4-nitrophenyl)-2,2-dichloro- 52335-34-9 C15H10Cl2N2O4
Engel net impurity 20 Engel net impurity 20 2452301-18-5 C23H29ClO8
(3R,4R)-3-((benzo[d][1,3]dioxol-5-yloxy)methyl)-4-(4-fluorophenyl)-1-methylpiperidine (3R,4R)-3-((benzo[d][1,3]dioxol-5-yloxy)methyl)-4-(4-fluorophenyl)-1-methylpiperidine
Imidapril Impurity Imidapril Impurity
Benzamide, 4-[3-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-5,5-dimethyl-4-oxo-1-imidazolidinyl]-2-fluoro-N-methyl- Benzamide, 4-[3-[4-cyano-3-(trifluoromethyl)phenyl]-2-hydroxy-5,5-dimethyl-4-oxo-1-imidazolidinyl]-2-fluoro-N-methyl- 2029195-97-7 C21H18F4N4O3
Ergoline-8-methanol, 10-methoxy-6-methyl-, (8β,10β)- (9CI) Ergoline-8-methanol, 10-methoxy-6-methyl-, (8β,10β)- (9CI) 201931-22-8 C17H22N2O2
(S)-3(2h)-Pyridazinone, 6-(4-Aminophenyl)-4,5-Dihydro-5-Methyl- (S)-3(2h)-Pyridazinone, 6-(4-Aminophenyl)-4,5-Dihydro-5-Methyl- 101328-84-1 C11H13N3O
2-Chloro-4-nitrobenzaldehyde 2-Chloro-4-nitrobenzaldehyde 5568-33-2 C7H4ClNO3
Bromhexine Glucose Compound 2 Bromhexine Glucose Compound 2
(S)-4-Desmethoxy-4-chloro Omeprazole (S)-4-Desmethoxy-4-chloro Omeprazole 863029-89-4 C16H16ClN3O2S
CISAPRIDEMONOHYDRATE CISAPRIDEMONOHYDRATE 260779-88-2 C23H31ClFN3O5
3,17-bis-deacetylvecuronium 3,17-bis-deacetylvecuronium 73319-30-9 C30H53BrN2O2
1,8,1',8'-tetrahydroxybisanthrone 1,8,1',8'-tetrahydroxybisanthrone 31991-54-5 C28H18O6
17-Desacetyl Rocuronium Bromide 17-Desacetyl Rocuronium Bromide 119302-86-2 C30H51BrN2O3
platycoside E platycoside E 237068-41-6 C69H112O38
HYOCHOLIC ACID HYOCHOLIC ACID 547-75-1 C24H40O5
Citalopram N-Oxide Citalopram N-Oxide 63284-72-0 C20H21FN2O2
7-epi-Clindamycin 7-epi-Clindamycin 16684-06-3 C18H33ClN2O5S
Aciclovir IMp. D (EP) Aciclovir IMp. D (EP) 59277-91-7 C15H15N5O4
Fulvestrant Sterol DiMer Fulvestrant Sterol DiMer
Lapatinib IMpurity 8 Lapatinib IMpurity 8 320337-48-2 C26H19ClFN3O3
2-[3-(AMinocarbonyl)-4-(2-Methylpropoxy)phenyl]-4-Methyl-5-thiazolecarboxylic Acid 2-[3-(AMinocarbonyl)-4-(2-Methylpropoxy)phenyl]-4-Methyl-5-thiazolecarboxylic Acid 1239233-86-3 C16H18N2O4S
2-Pyrrolidinyl-3-acetyl Desmorpholinylrocuronium Bromide 2-Pyrrolidinyl-3-acetyl Desmorpholinylrocuronium Bromide 1190105-66-8 C34H55BrN2O4
Afatinib IMpurity E Afatinib IMpurity E
Brivaracetam (alfaR, 4S)-Isomer Brivaracetam (alfaR, 4S)-Isomer 357336-99-3 C11H20N2O2
Entacapone EP Impurity G Entacapone EP Impurity G 1364322-41-7 C11H9N3O5
Isogambogenic acid Isogambogenic acid 887923-47-9 C38H46O8
1-Ethyl-3-[[4-(m-toluidino)-3-pyridyl]sulfonyl]urea 1-Ethyl-3-[[4-(m-toluidino)-3-pyridyl]sulfonyl]urea 58155-35-4 C15H18N4O3S
Atorvastatin N-(3,5-Dihydroxy-7-heptanoic Acid)amide Atorvastatin N-(3,5-Dihydroxy-7-heptanoic Acid)amide 1105067-87-5 C40H50CaFN3O8
3-[2-(Diaminomethyleneamino)-1,3-thiazol-4-ylmethylsulphinyl]-N-sulphamoyl
propanamide 3-[2-(Diaminomethyleneamino)-1,3-thiazol-4-ylmethylsulphinyl]-N-sulphamoyl propanamide 1020719-36-1 C8H14N6O4S3
(1S,3S)-1-(1,3-Benzodioxol-5-yl)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic Acid Methyl Ester (1S,3S)-1-(1,3-Benzodioxol-5-yl)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic Acid Methyl Ester 171596-43-3 C20H18N2O4
16,17-Dehydro Capsaicin 16,17-Dehydro Capsaicin 509101-57-9 C18H25NO3
1,1'-[(5E)-5-Methyl-7-oxo-5-undecene-1,11-diyl] Bis 1,1'-[(5E)-5-Methyl-7-oxo-5-undecene-1,11-diyl] Bis 874747-30-5 C26H34N8O5
16-Oxo Ethynyl Estradiol 16-Oxo Ethynyl Estradiol 1350468-76-6 C20H22O3
Uridine Diphosphate Choline Uridine Diphosphate Choline 99492-83-8 C14H25N3O12P2
2-ChloroMethyl-4-Methoxy-3,5-diMethylpyridine 1-Oxide 2-ChloroMethyl-4-Methoxy-3,5-diMethylpyridine 1-Oxide 848694-10-0 C9H12ClNO2
2-ChloroMethyl-4-Methoxy-3,5-diMethylpyridine 1-Oxide 2-ChloroMethyl-4-Methoxy-3,5-diMethylpyridine 1-Oxide 848694-10-0 C9H12ClNO2
(R,R,R)-Aprepitant (R,R,R)-Aprepitant 1148113-53-4 C23H21F7N4O3
Rufinamide Related Compound B (25 mg) (Methyl 1-(2,6-difluorobenzyl)-1H-1,2,3-triazole-4-carboxylate) Rufinamide Related Compound B (25 mg) (Methyl 1-(2,6-difluorobenzyl)-1H-1,2,3-triazole-4-carboxylate) 217448-86-7 C11H9F2N3O2
3,4-Dihydro-7-(4-hydroxybutoxy)-2(1H)-quinolinone 3,4-Dihydro-7-(4-hydroxybutoxy)-2(1H)-quinolinone 889443-20-3 C13H17NO3
cefotaxiMe IMpurity D cefotaxiMe IMpurity D 63527-53-7 C16H17N5O7S2
ZAFIRLUKAST IMpurity – D ZAFIRLUKAST IMpurity – D 1160235-24-4 C46H49N5O8S
Afatinib iMpurity Afatinib iMpurity 1680184-59-1 C24H25ClFN5O3
Levocarnitine iMpurity Levocarnitine iMpurity 6490-20-6 C7H17ClN2O2
(R)-9,10-difluoro-3-methyl-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid (R)-9,10-difluoro-3-methyl-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid 110548-07-7 C13H9F2NO4
(7R)-3-methyl-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (7R)-3-methyl-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid 34691-02-6 C14H14N2O4S2
Olmesartan Medoxomil Impurity 4 Olmesartan Medoxomil Impurity 4 95579-71-8 C5H5ClO3
4-methoxy-3-(3-morpholinopropoxy)-2-nitrobenzonitrile 4-methoxy-3-(3-morpholinopropoxy)-2-nitrobenzonitrile 1006378-06-8 C15H19N3O5
5-methoxy-2-((4-methoxy-3,5-dimethylpyridin-2-yl)methyl)-1H-benzo[d]imidazole 5-methoxy-2-((4-methoxy-3,5-dimethylpyridin-2-yl)methyl)-1H-benzo[d]imidazole 1422064-00-3 C9H12ClNO2S
Metronidazole Impurity 9 Metronidazole Impurity 9 87009-72-1 C13H13N3O4
Ganciclovir EP impurity A Ganciclovir EP impurity A 1797982-93-4 C9H10ClN5O2
8-methyl-2-(4-methylphenyl)imidazo[1,2-a]pyridine 8-methyl-2-(4-methylphenyl)imidazo[1,2-a]pyridine 381717-12-0 C15H14N2
4-[2-(tert-butylamino)ethyl]-2-(hydroxymethyl)phenol 4-[2-(tert-butylamino)ethyl]-2-(hydroxymethyl)phenol 1823256-56-9 C13H21NO2
3-[2-[4-(6-fluoro-1,2-benzoxazol-3-yl)piperidin-1-yl]ethyl]-9-hydroxy-2-methylpyrido[1,2-a]pyrimidin-4-one 3-[2-[4-(6-fluoro-1,2-benzoxazol-3-yl)piperidin-1-yl]ethyl]-9-hydroxy-2-methylpyrido[1,2-a]pyrimidin-4-one 766485-15-8 C23H23FN4O3
(E)-methyl 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylate (E)-methyl 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylate 956932-46-0 C14H14N2O2
(E)-methyl 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylate (E)-methyl 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylate 956932-46-0 C14H14N2O2
2-(3-methylbutanoyl)benzoic acid 2-(3-methylbutanoyl)benzoic acid 152567-80-1 C12H14O3
Eltrombopag Dimer Impurity Eltrombopag Dimer Impurity
ethyl 2-(3,5-diformyl-4-hydroxyphenyl)-4-methylthiazole-5- carboxylate ethyl 2-(3,5-diformyl-4-hydroxyphenyl)-4-methylthiazole-5- carboxylate 2073891-59-3 C15H13NO5S
Amlodipine Impurity 26 Amlodipine Impurity 26 107812-86-2 C18H19Cl2NO4
Terbutaline EP Impurity B Hemisulphate Terbutaline EP Impurity B Hemisulphate 153657-83-1 C13H21NO7S
Deferasirox Impurity 8 Deferasirox Impurity 8 201530-78-1 C21H15N3O4
Carbidopa Impurity Carbidopa Impurity 1176784-51-2 C9H8N2O2
Carbocisteine Impurity 5 Carbocisteine Impurity 5 118492-49-2 C5H7NO4S
Zolpidem Impurity 35 Zolpidem Impurity 35 1186313-33-6 C31H28N4
(trans)-3-((benzo[d][1,3]dioxol-5-yloxy)methyl)-4-(4'-fluoro-[1,1'- biphenyl]-3-yl)-1-methylpiperidine hydrochloride (trans)-3-((benzo[d][1,3]dioxol-5-yloxy)methyl)-4-(4'-fluoro-[1,1'- biphenyl]-3-yl)-1-methylpiperidine hydrochloride C26H27ClFNO3
Lacosamide impurity 8/Lacosamide EP Impurity H/(S)-2-acetamido-N-((S)-1-(benzylamino)-3-methoxy-1-oxopropan-2-yl)-3-methoxypropanamide Lacosamide impurity 8/Lacosamide EP Impurity H/(S)-2-acetamido-N-((S)-1-(benzylamino)-3-methoxy-1-oxopropan-2-yl)-3-methoxypropanamide 1375090-19-9 C17H25N3O5
Dapoxetine impurity 11/(S)-N-Didemethyl Dapoxetine HCl/(S)-α-[2-(1-Naphthalenyloxy)ethyl]benzenemethanamine Hydrochloride Dapoxetine impurity 11/(S)-N-Didemethyl Dapoxetine HCl/(S)-α-[2-(1-Naphthalenyloxy)ethyl]benzenemethanamine Hydrochloride 2095189-99-2 C19H20ClNO
Lifitegrast Impurity 14 Lifitegrast Impurity 14 2295862-24-5 C31H28Cl2N2O7S
(S)-4-(((R)-1-carboxy-2-((3-hydroxypropyl)
thio)ethyl)amino)-3-methyl-4-oxobutanoic 
acid (S)-4-(((R)-1-carboxy-2-((3-hydroxypropyl) thio)ethyl)amino)-3-methyl-4-oxobutanoic acid
Aprepitant EP Impurity C Aprepitant EP Impurity C 2348441-63-2 C29H25F7N4O3
Ergoline-8-methanol, 10-methoxy-1-methyl-, 5-bromo-3-pyridinecarboxylate (ester), (8β)- (9CI) Ergoline-8-methanol, 10-methoxy-1-methyl-, 5-bromo-3-pyridinecarboxylate (ester), (8β)- (9CI) 192504-81-7 C23H24BrN3O3
Ampicillin Glycolylurea Analogue Ampicillin Glycolylurea Analogue
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