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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride 911-77-3 C19H23ClN6O4
amphotericin A amphotericin A 1405-32-9 C47H75NO17
OPIACE reference substance OPIACE reference substance
OPIACE reference substance OPIACE reference substance
Peramivir Dehydration Deacetylation Impurity Peramivir Dehydration Deacetylation Impurity
Piperacillin Impurity S(EP) Piperacillin Impurity S(EP)
Curcumenol Curcumenol 19431-84-6 C15H22O2
spantide II spantide II 129176-97-2 C86H104Cl2N18O13
Phenol, 4,4′-sulfonylbis-, Polymer mit 1,1′-Oxibis[2-chlorethan],  EO 2-12 mol (75 % EO 2-6 mol) Phenol, 4,4′-sulfonylbis-, Polymer mit 1,1′-Oxibis[2-chlorethan], EO 2-12 mol (75 % EO 2-6 mol) 191680-83-8 (C12H10O4S.C4H8Cl2O)x
Aids004768 Aids004768 145986-07-8 C8H10N2O4S
ethyl 1-(4-methoxyphenyl)-6-(4-nitrophenyl)-7-oxo-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxylate ethyl 1-(4-methoxyphenyl)-6-(4-nitrophenyl)-7-oxo-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxylate 536759-91-8 C22H20N4O6
Dimethyl 4-(2-Chlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate Dimethyl 4-(2-Chlorophenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate 43067-01-2 C17H18ClNO4
cis-Terbinafine Hydrochloride cis-Terbinafine Hydrochloride 176168-78-8 C21H26ClN
Clindamycin B Clindamycin B 18323-43-8 C17H31ClN2O5S
Pregna-4,14-diene-3,20-dione Pregna-4,14-diene-3,20-dione 24377-08-0 C21H28O2
(6R,7R)-7-Amino-3-[(5-methyl-2H-tetrazol-2-yl)methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (6R,7R)-7-Amino-3-[(5-methyl-2H-tetrazol-2-yl)methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid 82549-51-7 C10H12N6O3S
Boceprevir InterMediates Boceprevir InterMediates 565456-77-1 C9H16ClNO2
3''-HABA KanaMycin A 3''-HABA KanaMycin A 50725-25-2 C22H43N5O13
(3β,20S)-20-Formyl-3-hydroxy-5-pregnene 3-O-Acetate (3β,20S)-20-Formyl-3-hydroxy-5-pregnene 3-O-Acetate 10211-88-8 C24H36O3
3-Desacetyl CefotaxiMe Lactone 3-Desacetyl CefotaxiMe Lactone 66340-33-8 C14H13N5O5S2
21-Dehydro Budesonide 21-Dehydro Budesonide 85234-63-5 C25H32O6
Levalbuterol Related Compound B (20 mg) (alpha-[{(1,1-Dimethylethyl)amino}methyl]-4-hydroxy-3-methyl-benzenemethanol) Levalbuterol Related Compound B (20 mg) (alpha-[{(1,1-Dimethylethyl)amino}methyl]-4-hydroxy-3-methyl-benzenemethanol) 18910-68-4 C13H21NO2
3-O-De(2,6-dideoxy-3-C-Methyl-3-O-Methyl-α-L-ribo-hexopyranosyl)-8,9,10,11-tetradehydro-9-deoxo-11,12-dideoxy-9,12-epoxy-6-O-MethylerythroMycin 3-O-De(2,6-dideoxy-3-C-Methyl-3-O-Methyl-α-L-ribo-hexopyranosyl)-8,9,10,11-tetradehydro-9-deoxo-11,12-dideoxy-9,12-epoxy-6-O-MethylerythroMycin 127157-35-1 C30H51NO8
7-(4-BroMobenzoyl)- 7-(4-BroMobenzoyl)- 241825-88-7 C15H8BrNO3
Valdecoxib IMpurity B Valdecoxib IMpurity B 1373038-60-8 C32H25N3O6S2
2-(((4-(3-methoxypropoxy)-3-methylpyridin-2-yl)methyl)thio)-1H-benzo[d]imidazole 2-(((4-(3-methoxypropoxy)-3-methylpyridin-2-yl)methyl)thio)-1H-benzo[d]imidazole C18H21N3O2S
bortezoMib iMpurity C bortezoMib iMpurity C
cefotaxiMe IMpurity F cefotaxiMe IMpurity F
5-ForMyl-2-Methoxy-benzenesulfonaMide 5-ForMyl-2-Methoxy-benzenesulfonaMide 105764-07-6 C8H9NO4S
3'-Desethoxy-drotaverine 3'-Desethoxy-drotaverine 85475-88-3 C22H27NO4
8-EpitacroliMus 8-EpitacroliMus 129212-35-7 C44H69NO12
Xylohexaose Xylohexaose 49694-21-5 C30H50O25
Avibactam INT 1 Avibactam INT 1 1416134-48-9 C17H24N2O7
(1S,3R,4S)-4-(2-((5-chloropyridin-
2-yl)amino)-2-oxoacetamido)-3-(5-methyl-4,5,6,7-tetrahydrothiazolo[5,4-c]pyridine-2-carboxamido)cyclohexane-1-carboxylic acid (1S,3R,4S)-4-(2-((5-chloropyridin- 2-yl)amino)-2-oxoacetamido)-3-(5-methyl-4,5,6,7-tetrahydrothiazolo[5,4-c]pyridine-2-carboxamido)cyclohexane-1-carboxylic acid 834919-19-6 C22H25ClN6O5S
Iguratimod Impurity 10 Iguratimod Impurity 10 149456-97-3 C18H20N2O6S
Valsartan Impurity 32 Valsartan Impurity 32 152708-24-2 C14H11N7
Apremilast Impurity 28 Apremilast Impurity 28 1802246-60-1 C30H27N3O9S
(2R,4R)-4-methyl-1-(((3-methylquinolin-8-yl)sulfonyl)-L-arginyl)piperidine-2-carboxylic acid (2R,4R)-4-methyl-1-(((3-methylquinolin-8-yl)sulfonyl)-L-arginyl)piperidine-2-carboxylic acid 951130-92-0 C23H32N6O5S
Minodronic Acid Impurity 2 Minodronic Acid Impurity 2 127657-45-8 C9H12N2O7P2
isopropyl ((S)-((((R)-1-(6-amino-9H-purin-9-yl)propan-2-yl)oxy)methyl)(phenoxy)phosphoryl)-D-alaninate isopropyl ((S)-((((R)-1-(6-amino-9H-purin-9-yl)propan-2-yl)oxy)methyl)(phenoxy)phosphoryl)-D-alaninate 2053424-82-9 C21H29N6O5P
Apixaban Impurity BMS-591329 Apixaban Impurity BMS-591329 2029205-64-7 C24H22ClN5O3
Dapagliflozin Impurity 21 Dapagliflozin Impurity 21
Regorafenib impurity H Regorafenib impurity H 2141961-84-2 C15H14FN3O3
Cefditoren Pivoxil Dimer Cefditoren Pivoxil Dimer
UFFLDXFQXNYZLF-BHRSBNONSA-N UFFLDXFQXNYZLF-BHRSBNONSA-N 2084828-53-3 C52H61N7O8S2
6,6′-bis(hydroxymethyl)-2,2′-bipyridine 6,6′-bis(hydroxymethyl)-2,2′-bipyridine 74065-63-7 C12H12N2O2
Chitobiose 2HCl Chitobiose 2HCl 577-76-4 C12H24N2O9
Rocuronium bromide Impurity 32 Rocuronium bromide Impurity 32
Seco Rapamycin Seco Rapamycin 147438-27-5 C51H79NO13
Lapatinib 4-Fluoro Impurity Lapatinib 4-Fluoro Impurity 1026818-86-9 C29H26ClFN4O4S
Arbidol Impurity 1 Arbidol Impurity 1 1130901-04-0 C21H23BrN2O3S
Difluprednate Impurity 11 Difluprednate Impurity 11 1270039-29-6 C21H24F2O4
Ozagrel impurity D Ozagrel impurity D
Pramipexole Impurity 13 HCl Pramipexole Impurity 13 HCl
Albendazole EP Impurity J Albendazole EP Impurity J 946498-41-5 C9H7Cl2N3O2
Avibactam Impurity 46 Avibactam Impurity 46 1804915-68-1 C7H10N3NaO5S
Cilastatin Impurity F Cilastatin Impurity F
Teriflunomide Impurity 1 Teriflunomide Impurity 1 1385789-70-7 C12H12N2O2
Atorvastatin Calcium Hydrate impurity 33 Atorvastatin Calcium Hydrate impurity 33 2299304-89-3 C24H44N2O7
1-cyclopropyl-8-fluoro-6-methoxy-7-((4aS,7aS)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid 1-cyclopropyl-8-fluoro-6-methoxy-7-((4aS,7aS)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid 2252446-69-6 C21H25ClFN3O4
N-DEMETHYL ERYTHROMYCIN A N-DEMETHYL ERYTHROMYCIN A 992-62-1 C36H65NO13
Ritonavir EP Impurity B(HCl) Ritonavir EP Impurity B(HCl)
Butenafine Impurity 12 Butenafine Impurity 12 759458-92-9 C13H13NO
△3-Cefoperazone △3-Cefoperazone
Peramivir Impurity 31 Peramivir Impurity 31 2758048-80-3 C15H28N2O4
(R)-2-TERT-BUTYLOXYCARBONYLAMINO-PENTANE-1,5-DIOL (R)-2-TERT-BUTYLOXYCARBONYLAMINO-PENTANE-1,5-DIOL 397246-12-7 C10H21NO4
15(R)-15-METHYL PROSTAGLANDIN F2ALPHA 15(R)-15-METHYL PROSTAGLANDIN F2ALPHA 35864-81-4 C21H36O5
BROMOCLENBUTEROL BROMOCLENBUTEROL 37153-52-9 C12H18BrClN2O
2,7,10,12-Tetraazatridecanoic acid, 4-hydroxy-12-methyl-9-(1-methylethyl)-13-[2-(1-methylethyl)-4-thiazolyl]-8,11-dioxo-3,6-bis(phenylmethyl)-, 5-thiazolylmethyl ester, (3S,4R,6R,9S)- 2,7,10,12-Tetraazatridecanoic acid, 4-hydroxy-12-methyl-9-(1-methylethyl)-13-[2-(1-methylethyl)-4-thiazolyl]-8,11-dioxo-3,6-bis(phenylmethyl)-, 5-thiazolylmethyl ester, (3S,4R,6R,9S)- 1414933-82-6 C37H48N6O5S2
1,2,4-Oxadiazole-3-carboxamide, N-(3-chloro-2-hydroxypropyl)-5-methyl- 1,2,4-Oxadiazole-3-carboxamide, N-(3-chloro-2-hydroxypropyl)-5-methyl- 110578-70-6 C7H10ClN3O3
Pentoxifylline Impurity Pentoxifylline Impurity 1243654-85-4 C13H20N4O6
1H-1,2,3-Triazole-4-methanol, 1-[(2,6-difluorophenyl)methyl]- 1H-1,2,3-Triazole-4-methanol, 1-[(2,6-difluorophenyl)methyl]- 1364193-40-7 C10H9F2N3O
(E)-5-methoxy-1-[4-(trifluoromethyl)phenyl]pentan-1-one oxime (E)-5-methoxy-1-[4-(trifluoromethyl)phenyl]pentan-1-one oxime 88699-84-7 C13H16F3NO2
Flomoxef Impurity III Flomoxef Impurity III
Ampicillin Impurity Q(EP)(a trimer )Or Impurity M n=2 Ampicillin Impurity Q(EP)(a trimer )Or Impurity M n=2
Cyclopenta[5,6]naphtho[2,1-b]pyran-7-carboxylic acid, 2,3,4,4a,4b,5,6,6a,7,8,9,9a,9b,10-tetradecahydro-4a,6a-dimethyl-2-oxo-, methyl ester, (4aR,4bS,6aS,7S,9aS,9bS)- Cyclopenta[5,6]naphtho[2,1-b]pyran-7-carboxylic acid, 2,3,4,4a,4b,5,6,6a,7,8,9,9a,9b,10-tetradecahydro-4a,6a-dimethyl-2-oxo-, methyl ester, (4aR,4bS,6aS,7S,9aS,9bS)- 153222-81-2 C20H28O4
2-(4-(2-(4-(1H-benzo[d]imidazol-2-yl)piperidin-1-yl)ethyl)phenyl)-2-methylpropanoic acid 2-(4-(2-(4-(1H-benzo[d]imidazol-2-yl)piperidin-1-yl)ethyl)phenyl)-2-methylpropanoic acid C24H29N3O2
Rapamycin, 32-O-demethyl- Rapamycin, 32-O-demethyl- 141392-23-6 C50H77NO13
11-monoketofusidic acid 11-monoketofusidic acid 16711-91-4 C31H46O6
(3R,5S,5'aR)-5-(3-Furyl)-3',4,5,5',5'aα,7',8',8'aα-octahydro-7'α-methylspiro[furan-3(2H),6'-[6H]naphtho[1,8-bc]furan]-2,2'(4'H)-dione (3R,5S,5'aR)-5-(3-Furyl)-3',4,5,5',5'aα,7',8',8'aα-octahydro-7'α-methylspiro[furan-3(2H),6'-[6H]naphtho[1,8-bc]furan]-2,2'(4'H)-dione 53625-15-3 C19H20O5
(+)-Bisabolangelone (+)-Bisabolangelone 30557-81-4 C15H20O3
Acotiamide Hydrochloride Acotiamide Hydrochloride 185104-11-4 C21H31ClN4O5S
(S)-N-(1-aMino-1-oxobutan-2-yl)-4-chlorobutanaMide (S)-N-(1-aMino-1-oxobutan-2-yl)-4-chlorobutanaMide 102767-31-7 C8H15ClN2O2
[3-[[[2-(DiaMinoMethyleneaMino)-4-thiazolyl]Methyl]thio]propionyl]sulfaMide Hydrochloride (FaMotidine IMpurity) [3-[[[2-(DiaMinoMethyleneaMino)-4-thiazolyl]Methyl]thio]propionyl]sulfaMide Hydrochloride (FaMotidine IMpurity) 76824-17-4 C8H15ClN6O3S3
Defluoro Aprepitant Defluoro Aprepitant 170729-76-7 C23H22F6N4O3
[3S-[2(R*),3α,8aβ]]-Hexahydro-3-Methyl-1,4-dioxo-α-(2-phenylethyl)pyrrolo[1,2-a]pyrazine-2(1H)-acetic Acid [3S-[2(R*),3α,8aβ]]-Hexahydro-3-Methyl-1,4-dioxo-α-(2-phenylethyl)pyrrolo[1,2-a]pyrazine-2(1H)-acetic Acid 115623-21-7 C18H22N2O4
N-(3,5-DiMethyltricyclo[3.3.1.13,7]dec-1-yl)glycine N-(3,5-DiMethyltricyclo[3.3.1.13,7]dec-1-yl)glycine 1340545-88-1 C14H23NO2
Tenuifoliside A Tenuifoliside A 139726-35-5 C31H38O17
Gefitinib iMpurity Gefitinib iMpurity 1608115-59-8 C22H23ClFN3O4
AMoxicillin IMpurity M AMoxicillin IMpurity M C16H19N3O5S
Apixaban IMpurity 3 Apixaban IMpurity 3 1801881-18-4 C25H25N5O4
TaMsulosin EP IMpurity A TaMsulosin EP IMpurity A 918867-88-6 C30H40N2O7S
Forsythoside I Forsythoside I 1177581-50-8 C29H36O15
2-cyanoethyl 3-aminobut-2-enoate 2-cyanoethyl 3-aminobut-2-enoate 193539-61-6 C7H10N2O2
4,4'-Bis-Dimethylsilyl-Biphenyl 4,4'-Bis-Dimethylsilyl-Biphenyl 17937-85-8 C16H22Si2
2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5-carboxylic acid 2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5-carboxylic acid 1530308-87-2 C15H14N2O3S
4-bromo-1-chloro-2-(2-ethoxybenzyl)benzene 4-bromo-1-chloro-2-(2-ethoxybenzyl)benzene 1830346-16-1 C15H14BrClO
Clopidogrel EP Impurity B HCl Clopidogrel EP Impurity B HCl 1396607-35-4 C16H17Cl2NO2S
Saikosaponin B3 Saikosaponin B3 58316-42-0 C43H72O14
diethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate diethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 21829-26-5 C19H22N2O6
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