一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Frontiers in Physiology >>article
Frontiers in Physiology

Frontiers in Physiology

IF: 3.2
Download PDF

Gluconolactone Alleviates Myocardial Ischemia/Reperfusion Injury and Arrhythmias via Activating PKCε/Extracellular Signal-Regulated Kinase Signaling

Published:14 March 2022 DOI: 10.3389/fphys.2022.856699
Xinghua Qin,?Bin Liu,?F. Gao,?Yuanyuan Hu,?Ziwei Chen,?Jie Xu,?Xing Zhang

Abstract

Gluconolactone (D-glucono-1,5-lactone or GDL) is a food additive which presents in dietary products such as tofu, yogurt, cheese, bread, wine, etc. GDL has long been considered as a free radical scavenger; however, its role in cardioprotection remains elusive. In this study, using a mouse model of myocardial ischemia/reperfusion (I/R) injury and a model of hypoxia/reoxygenation (H/R) in neonatal rat cardiomyocytes (NRCM), we explored the role of GDL in I/R injury. We found that GDL (5 mg/kg, i.p.) attenuated myocardial I/R injury as evidenced by decreased infarct size, release of cardiac injury markers and apoptosis. Additionally, GDL decreased reperfusion-induced arrhythmias and oxidative stress. These effects were also observed in parallel in vitro studies. Mechanistically, we found that GDL treatment was strongly associated with activation of pro-survival extracellular signal-regulated kinase (ERK) signaling both in vivo and in vitro, and pharmacological inhibition of ERK signaling via U0126 attenuated GDL-induced cardioprotection against H/R injury in NRCM cells. To reveal how GDL regulates ERK signaling, we predicted the putative targets of GDL by Swiss Target Prediction, and protein kinase C (PKC) emerged as the most promising target for GDL. By pharmacological intervention and immunofluorescence, we found that PKCε, an important member of the PKC family, was activated after GDL treatment in heart, thereby leading to ERK activation and cardioprotection against I/R injury. Taken together, our results demonstrated that GDL acts as a potent activator of PKCε and, thus, provides cardioprotection against I/R injury via activation of ERK signaling.

Substances (11)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
Chelerythrine 34316-15-9 C21H18NO4+ 254 suppliers $40.00-$2395.30
Chelerythrine 34316-15-9 C21H18NO4+ 254 suppliers $40.00-$2395.30
Chelerythrine 34316-15-9 C21H18NO4+ 254 suppliers $40.00-$2395.30
Chelerythrine 34316-15-9 C21H18NO4+ 254 suppliers $40.00-$2395.30
ROTTLERIN 82-08-6 C30H28O8 167 suppliers $40.00-$1885.68
ROTTLERIN 82-08-6 C30H28O8 167 suppliers $40.00-$1885.68
ROTTLERIN 82-08-6 C30H28O8 167 suppliers $40.00-$1885.68
ROTTLERIN 82-08-6 C30H28O8 167 suppliers $40.00-$1885.68
依安县| 商南县| 抚顺县| 苗栗市| 中宁县| 福海县| 嵊州市| 临朐县| 旌德县| 安平县| 庆城县| 汝南县| 昌图县| 昌图县| 延川县| 丹东市| 隆安县| 前郭尔| 班玛县| 修武县| 张家川| 柘荣县| 内乡县| 谷城县| 昌都县| 临沭县| 丰台区| 土默特右旗| 英吉沙县| 广饶县| 古浪县| 中牟县| 康马县| 凤庆县| 皋兰县| 黎平县| 宜都市| 石城县| 光泽县| 乐安县| 苗栗县|